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Vitamin B Rich Folates Significantly Reduce Alzheimer’s Disease Risk

   
  Leafy greens, dried beans and peas, fortified cereals and grain products, and some fruits and vegetables are  rich sources of folate.  

Beats antioxidants, like vitamin E, and other nutrients for health of aging brain in study of senior citizens

Aug. 12, 2005- A study of senior citizens says those who eat the daily recommended allowance of folates – B vitamin nutrients found in oranges, legumes, leafy green vegetables and folic acid supplements – “significantly reduce” their risk of developing Alzheimer’s disease.

The study, a long-term look at diet and brain aging by the National Institute on Aging, also found that folates appear to have more impact on reducing Alzheimer's risk than vitamin E, a noted antioxidant, and other nutrients considered for their effect as a brain-aging deterrent.

 

Finding the Folate in Foods

 
 

Agricultural Research Service scientists at the Food Composition Laboratory (FCL), in Beltsville, Maryland, have developed a new method to analyze the essential B vitamin folate in foods and in blood serum.

Folate piqued America's nutritional interest when the Food and Drug Administration in 1998 required that grain products be fortified with it. Evidence showed that risks of birth defects would drop if mothers-to-be took more folic acid.

Folate is important to white blood cell makeup and for regulation of the amino acid homocysteine. Folate is also involved in nucleic acid synthesis and methylation reactions, both of which help the body form genetic material, or DNA.

"Dietary folate is important for all aspects of life—especially during growth," says Robert J. Pawlosky, the FCL chemist who first reported the new folate analysis method last year. "It also appears to be important for maintaining cardiovascular tone and preventing heart disease."

Folate is a generic term used for a family of related compounds that exhibit similar vitamin activity within the body. The family includes folic acid, which is the major synthetic form of folate used by food processors to fortify foods. Each is absorbed by the body at different rates.

The new method for analyzing the amount of folate in foods uses high-performance liquid chromatography and mass spectrometry, or HPLC-MS. Current methods do not test for individual folates separately. But, says Pawlosky, "With the combination of HPLC-MS and stable isotopes, we can detect and measure very low levels of specific folates." The lab is now perfecting yet another way to analyze folate—HPLC with fluorescence detection. It's a less expensive method, but one that will complement the HPLC-MS method.

Accuracy is important to experts who establish and reexamine the nationally recommended folate levels and who monitor the folate fortification program. For example, in 1989, the recommended dietary allowance was lowered from 400 micrograms (mcg) to 200 mcg for men and 180 mcg for women. Then, in 1998, dietary folate equivalents were established—because of differences in bioavailability, or absorption—of at least 400 mcg per day for adults.

The ARS researchers now have a memorandum of understanding with the Nutrition Institute at the University of Chile at Santiago. Together, they are testing food and blood samples to gauge levels of folate and folic acid. Chile's program is designed to compare the country's fortification program of foods with how much they find in the Chilean population. Pawlosky says lessons from the program's design may eventually be used as a model for similar programs in the United States.

FCL scientists are also working with the National Institute of Standards and Technology (NIST) and the Centers for Disease Control and Prevention (CDC) to coordinate their assays of folate status in blood. The CDC monitors nutrition status across the country, and NIST develops assays for private technicians to use commercially in clinical labs.—By Rosalie Marion Bliss, Agricultural Research Service Information Staff.

This research is part of Human Nutrition, an ARS National Program at http://www.nps.ars.usda.gov.

Robert J. Pawlosky is with the USDA-ARS Food Composition Laboratory

"Finding the Folate in Foods" was published in the December 2002 issue of Agricultural Research magazine.

 

Ultimately, 57 of the original 579 participants in the study developed Alzheimer's disease. But the researchers found that those with higher intake of folates, vitamin E and vitamin B6 shared lower comparative rates of the disease. And when the three vitamins were analyzed together, only folates were associated with a significantly decreased risk.

In turn, no association was found between vitamin C, carotenoids (such as beta-carotene) or vitamin B-12 intake and decreased Alzheimer's risk.

Maria Corrada and Dr. Claudia Kawas of UC Irvine's Institute for Brain Aging and Dementia led the effort, which analyzed the diets of non-demented men and women age 60 and older.

"Although folates appear to be more beneficial than other nutrients, the primary message should be that overall healthy diets seem to have an impact on limiting Alzheimer's disease risk," said Corrada, who like Kawas started with the study while at Johns Hopkins University in Baltimore.

They compared the food nutrient and supplement intake of those who later developed Alzheimer's disease to the intake of those who did not develop the disease. It is the largest study to date to report on the association between folate intake and Alzheimer's risk and to analyze antioxidants and B vitamins simultaneously.

Results appear in the inaugural issue of the quarterly peer-reviewed research journal, Alzheimer's & Dementia: The Journal of the Alzheimer's Association.

The researchers used data from the Baltimore Longitudinal Study of Aging to identify the relationship between dietary factors and Alzheimer's disease risk. Between 1984 and 1991, study volunteers provided detailed dietary diaries, which included supplement intake and calorie amounts, for a typical seven-day period.

"The participants who had intakes at or above the 400-microgram recommended dietary allowance of folates had a 55-percent reduction in risk of developing Alzheimer's," said Corrada, an assistant professor of neurology. "But most people who reached that level did so by taking folic acid supplements, which suggests that many people do not get the recommended amounts of folates in their diets."

Folates have already been proven to reduce birth defects, and research suggests that they are beneficial to warding off heart disease and strokes.

Although folates are abundant in foods such as liver, kidneys, yeast, fruits (like bananas and oranges), leafy vegetables, whole-wheat bread, lima beans, eggs and milk, they are often destroyed by cooking or processing. Because of their link to reducing birth defects, folates have been added to grain products sold in the U.S. since 1998. But even with this supplement, it is thought that many Americans have folate-deficient diets.

Recent research is beginning to show relationships between folates and brain aging.

Earlier this year, Dutch scientists showed that adults who took 800 micrograms of folic acid daily had significant improved memory test scores, giving evidence that folates can slow cognitive decline.

"Given the observational nature of this study, it is still possible that other unmeasured factors also may be responsible for this reduction in risk," said Kawas, the Al and Trish Nichols Chair in Clinical Neuroscience. "People with a high intake of one nutrient are likely to have a high intake of several other nutrients and may generally have a healthy lifestyle. But further research and clinical studies on this subject will be necessary."

Judith Hallfrisch of the U.S. Department of Agriculture, Denis Muller with the National Institute on Aging and Ron Brookmeyer with Johns Hopkins collaborated on the study, which was originally undertaken at the Gerontology Research Center of the NIA and the Department of Neurology at Johns Hopkins. Study funding came from the Extramural Programs of the NIA.

Begun in 1958 by the NIA, the Baltimore Longitudinal Study of Aging is America's longest-running scientific study of human aging. BLSA scientists are learning what happens as people age and how to sort out changes due to aging from those due to disease or other causes. More than 1,400 men and women are study volunteers. For more information, see: www.grc.nia.nih.gov/branches/blsa/blsa.htm.

About the source:

About the University of California, Irvine: Celebrating 40 years of innovation, the University of California, Irvine is a top-ranked public university dedicated to research, scholarship and community service. Founded in 1965, UCI is among the fastest-growing University of California campuses, with more than 24,000 undergraduate and graduate students and about 1,400 faculty members. The second-largest employer in dynamic Orange County, UCI contributes an annual economic impact of $3 billion. For more UCI news, visit www.today.uci.edu.

UCI maintains an online directory of faculty available as experts to the media. To access, visit www.today.uci.edu/experts.

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